Run and Tumble

Diary of a bug farmer

Take away lesson from today’s lecture

Wednesday, October 7th, 2009

All water is poo water.  Unless you want your guts eaten, your brain eaten, or cysts in your tissues, don’t swim and stick to beer.

The Big Book of Bacteria

Thursday, October 1st, 2009

Tonight we’re going to be using Bergey’s Manual to identify some unknowns.   It’s basically the book for bacterial identification and classification. I’ve been wanting to get a look at Bergey’s since the first time I read about it, so I’m super jazzed for lab!

Forgive the length of my letter, I did not have time to write a short one

Tuesday, September 29th, 2009

(edit: This was written last Saturday, but I accidentally saved it as a draft, rather than publishing it.)
We were assigned an extra credit project, make an 8 to 15 slide presentation deck on a microbiology topic of our choice.
I’m doing mine on microbial bioremediation, of course. I’m amused that my biggest problem is figuring out [...]

Ted Park on discovering the method of action of penicillin

Tuesday, September 29th, 2009

Small Things Considered is running a great article wherein Ted Park recalls uncovering the way way penicillin works.
I find articles like this fascinating. It would be nice if there were more written experiences of “I didn’t know, but I had some hunches, so I tried this, this and this.  A colleague mentioned such-and-such and that [...]

Next test coming up

Tuesday, September 29th, 2009

Test #2 is slated for tomorrow. I’m all studied up. I don’t have any problems with the material. A few of the practice questions had subtly different answers where one was ‘kind of right’ and the other was ‘just a little righter’. If anything besides brainfarts or rushing trips me up, [...]

Bacterial noms

Friday, September 25th, 2009

Last night’s lab was spent learning about and inoculating more selective and differential media. We were mainly differentiating based on carbohydrate catabolism using fermentation tubes, MRVP broth, starch agar, OF-glucose media, and Simmon’s citrate. We’ll see the results on Tuesday.
We’re building up a toolbox of techniques we can use to work through a d-key and [...]

Lab Practical Results

Friday, September 25th, 2009

Remember the lab practical I was worried about?  I still think I should practice the areas I pointed out, but we got our grades back, and I did very well. So, that’s one less thing to worry about.

First lab practical done

Wednesday, September 23rd, 2009

Finished lab practical #1 last night. I think I did ok, but I did mess up a bit. Here’s where I’m predicting I lost points, and subsequently, what I’ve got to focus on.

Ran out of time before finishing 3rd streak on my streak plate. I’ve got to learn to work a little [...]

Winogradsky Column: Day 0

Monday, September 21st, 2009

I started two Winogradsky columns this past weekend.
They’re really simple to set up and were invented by Sergei  Winogradsky, one of the founding fathers of soil microbiology. The basic concept is that you get some mud and water, put it all in a semi-sealed tube exposed to light, and watch as bugs set up shop.
The [...]

Streaking: live nude bugs

Thursday, September 17th, 2009

We did streak plates earlier this week and I had a blast.
I’d been looking forward to doing a streak plate since before I even fully decided to take a class. Streaking is really basic and easy, but it elegantly solves one of the earliest problems in microbiology, “How do you isolate bacteria from the hodge [...]